软肝化坚颗粒对肝纤维化模型C57小鼠肝组织Bcl-2及Bax表达的调节作用

摘要

Objective To study the effect of Ruangan Huajian granule on the expressions of apoptosis related gene Bcl-2 and Bax in liver of C57 mice with experimental hepatic fibrosis. Methods Forty C57 mice were randomly divided into four groups: normal control group, model control group, low-dose of Ruangan Huajian granule group and high-dose of Ruangan Huajian granule group. There were 10 mice in every group. C57 mice with experimental hepatic fibrosis were induced by intraperitoneal injection of 10% CCl4. The expressions of Bcl-2 and Bax in liver tissues of mice were observed by immune histochemical technique. The score of fibrosis stages in the liver tissue was also analyzed. Results The expressions of Bcl-2 and Bax were mainly in the eosinophilic liver cells, especially in model control group. The eosinophilic liver cells were significantly reduced in low-dose and high-dose of Ruangan Huajian granule groups. The score of fibrosis stages and the positive expressions of Bcl-2 and Bax were significantly higher in model control group than those of normal control group (P < 0.05), but the value of Bcl-2/Bax was significantly lower in model control group than that of normal control group (P < 0.05). The positive expression of Bcl-2 was significantly higher in low-dose and high-dose of Ruangan Huajian granule groups than that of model control group and normal control group (P < 0.05). The positive expression of Bax was significantly lower in low-dose and high-dose of Ruangan Huajian granule groups than model control group, but it significantly enhanced than that of normal control group (P < 0.05). The value of Bcl-2/Bax was significantly higher than that of model control group and normal control group (P < 0.01).Conclusion Ruangan Huajian granule can modulate the expressions of Bcl-2 and Bax in liver tissues, and inhibit the liver cell apoptisis, showing an anti-fibrotic effect.%目的 研究软肝化坚颗粒对肝纤维化模型C57小鼠肝组织中凋亡调控相关基因Bcl-2及Bax表达的影响.方法 40只C57小鼠随机分为正常对照组、模型对照组、软肝化坚颗粒低剂量组及高剂量组,每组10只.采用四氯化碳腹腔注射诱导形成小鼠肝纤维化模型.采用免疫组织化学染色方法检测肝组织中Bcl-2和Bax的表达,同时进行肝组织纤维化评分.结果 Bcl-2和Bax均主要表达于中央静脉周围凝固性嗜酸性变肝细胞,模型对照组尤为明显,软肝化坚颗粒高、低剂量组凝固性嗜酸性变肝细胞明显减少.模型对照组肝组织肝纤维化评分、Bcl-2和Bax阳性表达量均显著高于正常对照组(P＜0.05),而Bcl-2/Bax比值显著低于正常对照组(P＜0.05).软肝化坚颗粒高、低剂量组肝组织Bcl-2阳性表达较模型对照组和正常对照组均明显增强(P＜0.05),Bax阳性表达量较模型对照组显著减少,但较正常对照组显著增强(P＜0.05),Bcl-2/Bax比值均显著高于模型对照组和正常对照组(P＜0.01).结论 软肝化坚颗粒能够调节小鼠肝细胞Bcl-2和Bax蛋白表达,阻断或抑制肝细胞凋亡.