艾灸预处理对脑缺血再灌注损伤模型大鼠大脑皮质caspase-3蛋白表达的影响

摘要

目的：观察艾灸预处理对脑缺血再灌注损伤模型大鼠大脑皮质半胱氨酸天冬氨酸蛋白酶（caspase）-3蛋白表达的影响，探讨艾灸预处理减轻脑缺血再灌注损伤的作用机制。方法雄性SD大鼠21只，分为假手术组(6只)、模型组(8只)和艾灸预处理组(7只)。采用线栓法闭塞大脑中动脉2 h后进行再灌注，制备局灶性脑缺血再灌注损伤大鼠模型。采用TTC染色检查脑组织梗死情况；HE染色检查大脑皮质的形态结构；电子显微镜观察大脑皮质神经细胞的超微结构；荧光免疫组织化学技术检测大脑皮质caspase-3蛋白的表达。结果艾灸预处理组大鼠患侧脑组织梗死体积百分比[(24.9±4.7)%]低于模型组[(45.8±4.6)%]，大脑皮质神经细胞形态结构变化较模型组轻，caspase-3阳性细胞数(39.17±7.28)低于模型组(58.17±16.53)。结论艾灸预处理具有减轻大鼠脑缺血再灌注损伤的作用，降低大脑皮质caspase-3蛋白的表达可能是其作用机制之一。%Objective To observe the effect of moxibustion pretreatment on caspase-3 expression in cortex of rats with cerebral ischemia-reperfusion injury. Methods Twenty-one male SD rats were randomly divided into three groups, sham group (n=6), model group (n=8) and moxibustion pretreatment group (n=7). Focal cerebral ischemia-reperfusion injury rat model was induced by middle cerebral artery occlusion (MCAO) for 2 hours followed by reperfusion. Brain infarct was ex-amined by TTC staining. Brain morphology was shown by HE staining and the ultrastructure of cell was observed with elec-tron microscope. Caspase-3 expression in cortex was examined by immunofluorescence histochemistry. Results The per-centage of infarct volume [(24.9±4.7)%] in moxibustion pretreatment group was much smaller than that [(45.8±4.6)%] in mod-el group. Nerve cells in moxibustion pretreatment group experienced less morphological and structural changes than those in model group, and caspase-3 positive cells (39.17 ± 7.28) in moxibustion pretreatment group were less than those (58.17 ± 16.53) in model group. Conclusion Moxibustion pretreatment could attenuate cerebral ischemia-reperfusion injury, which might be through decreasing caspase-3 expression.