环烯醚萜对烟酰胺-链脲霉素诱导的2型糖尿病小鼠生精功能的影响

摘要

目的：探讨环烯醚萜对烟酰胺-链脲霉素诱导的2型糖尿病小鼠生精功能的影响。方法56只雄性小鼠随机均分为7组（n=8）：对照组；糖尿病组；糖尿病小鼠服用格列本脲（0.25 mg/kg）治疗组；糖尿病小鼠接受环烯醚萜（20和40 mg/kg）治疗组；正常小鼠接受环烯醚萜（20和40 mg/kg）治疗组。糖尿病模型制作，由腹腔注射链脲霉素65 mg/kg，15 min后注射烟碱120 mg/kg，然后，连续喂服格列本脲和环烯醚萜28 d。最后1次治疗24 h后，提取各组血清样本、睾丸及附睾组织，测定血激素、观察睾丸病理切片、进行精子参数评估。结果环烯醚萜40 mg/kg和格列本脲治疗可以恢复糖尿病引起的小鼠体质量减少（P＜0.05）；糖尿病组小鼠附睾的精子浓度和存活率显著低于对照组，糖尿病+环烯醚萜40 mg/kg组的精子浓度和存活率显著高于糖尿病组，糖尿病+格列本脲组比糖尿病组的小鼠精子浓度增高（均P＜0.05）；糖尿病组小鼠与对照组相比血清黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）水平显著降低，环烯醚萜40 mg/kg和格列本脲可提升糖尿病动物的血清LH、FSH、T水平（均P＜0.05）。结论环烯醚萜可降低糖尿病小鼠氧化损伤的危重程度，有效提升其生育能力。%Objective To investigate the effects of iri doid on the spermatogenesis in mice of type-2 diabetes induced by nicotinamide and streptozotocin. Methods Fifty-six male C57BL mice were randomly divided into seven groups (n=8 for each group):control group, diabetic group, diabetic and glibenclamide (0.25 mg/kg) group, diabetic and iridoid (20 and 40 mg/kg) group, and iridoid (20 and 40 mg/kg) group. Diabetic model was induced by intraperitoneal injection of streptozotocin (65 mg/kg) and nicotinamide (120 mg/kg). Glibenclamide and iridoid were orally fed for 28 days. Twenty-four hours after the last treatment, blood samples, testis and the epididymis were collected for detecting blood hormones, testicular biopsy, and the evaluation of sperm parameters. Results The body weights of diabetic mice were recovered from the reduction after treatment with 40 mg/kg of iridoid and glibenclamide(P<0.05). The sperm count and viability were significantly decreased in diabetic group compared with those of control group (P<0.05). The sperm count was significantly increased in diabetic and glibenclamide group than that of diabetic group (P<0.05). The serum levels of lutein hormone (LH), follicle stimulating hormone (FSH) and testosterone (T) were significantly decreased in diabetic group compared with those of control group (P<0.05). The serum levels of LH, FSH and T were increased after treatment with 40 mg/kg of iridoid and glibenclamide (P<0.05). Conclusion These results suggest that iridoid can reduce the level of oxidative damage in diabetic mice, and effectively improve the fertility of mice.