塞来昔布对人结肠癌细胞系HT-29增殖的抑制及其对miRNA表达谱的影响

摘要

目的:研究塞来昔布对人结肠癌细胞系HT-29增殖的抑制作用及其对HT-29细胞微小核糖核酸(miRNA)表达谱的影响.方法 采用CCK-8比色还原法观察塞来昔布对HT-29细胞增殖的抑制作用；miRNA芯片技术检测塞来昔布作用前后HT-29细胞miRNA的表达变化,实时定量RT-PCR验证其结果.结果 塞来昔布对HT-29细胞增殖有显著的抑制作用,且呈剂量-时间效应关系,其半数抑制浓度为( 237.73±1.65) μmol/L;塞来昔布作用于HT-29细胞48 h后,获得28个与塞来昔布干预相关的miRNAs(P＜0.01),其中8个下调,20个上调.结论 塞来昔布对miRNA表达谱的影响可能是其抑制HT-29细胞株生长的作用途径之一.%Objective To study the anti-tumor effect of celecoxib on HT-29 colon cancer cell in vitro and its effect on the expression profiles of microRNA (miRNAs). Methods Human colon cancer cells HT-29 was treated by celecoxib in various concentrations alone. The inhibitory effect of cell growth was assayed by CCK-8 method. The expression profiles of miRNA was determined using miRNA microarray. The expression of selected miRNA was then validated by real-time RT-PCR. Results The inhibitory effect of celecoxib on the proliferation of HT-29 cell was found in does-and-time dependent manner. The IC50 value was (237.73 ± 1. 65) μmol/L, after 48 h, twenty-eight miRN A, 20 up-regulation and 8 down-regulation expression with statistical significance were identified in colon cancer cells following exposure to celecoxib compared to matched control cells. Conclusion Celecoxib could inhibit the HT-29 cell growth in vitro partly attributting to its effect on the miRNA expression.