红树植物老鼠簕总三萜含量的测定

摘要

老鼠簕是一种重要的具海洋药用价值的红树林植物。以分光光度法（齐墩果酸为对照品，5％香草醛－冰醋酸和高氯酸为显色剂）测定红树植物老鼠簕中总三萜的含量。实验结果表明：在500 nm波长条件下，齐墩果酸在0．04～0．20 mg・ mL －1范围内，0～60 min间，吸光度与含量线性关系良好；首次测定得到，红树植物老鼠簕根的总三萜含量为0．62％，（RSD＝0．91％，n ＝3）；茎的总三萜含量为2．20％（RSD＝3．94％，n ＝3）；叶的总三萜含量为1．59％（RSD＝5．04％，n＝3）。%Acanthus ilicifolius is an important mangrove plant which has medical values .Total triterpenoids in A-canthus ilicifolius was guantified by spectrometry method using oleanolic acid as standard and 5% vanillin-gIacial acetic acid& perchIoric acid as coIor-deveIoping agents.The resuIts showed that ,The content of the standard was well correlated to its optical density at 500 nm in the range of 0.04 ~0.20 mg・ mL-1 with a stability within 0 ~60min.Acanthus ilicifolius contained 0.62%(RSD=0.91%,n=3),2.20%(RSD=3.94%,n=3),1.59%(RSD=5.04%,n=3) of total triterpenoids in it′s root,stem and leaves according to the calculation of oleanolic acid con-tent.