Method for increasing the reserve capacity of nitrogen plants, nutritional value of forage or silage, the content of nitrogen in a plant or plant part, the construction of nucleotu00ecdeos, method for the production of a transformed plant and method for the determination of expre Breakthrough of zmlox in a plant tissue

摘要

Method for increasing the reserve capacity of nitrogen plants, nutritional value of forage or silage, the content of nitrogen in a plant or plant part, construction of nucleotideos,Method for the production of a transformed plant and method for the determination of the expression of zmlox in a plant tissue this invention provides methods and compositions for making and using transgenic plants with enhanced capacity to store nitrogen in comparau00e7u00e3 The wild plants.The methods of the invention comprise inducing super expression of vegetative protein reserves (VSPs) derivadasde monocotiledu00f3neas in plants, particularly in monocotiledu00f3neas. In some of the steps, at least one construction of nucleotides comprising the protein coding sequ00fau00eancia ZmLox6, or an active fragment or variant of this, inserted in a plant.Depending on the purpose, the construction of nucleotide can optionally include a coding sequ00fau00eancia operationally linked to a peptide or a peptide vacuolar tracking signal traffic to plastu00eddio so as to direct the storage of ZmLox6 protein, or a fragment, ITA VO, or variant of this in the compartments vacuolar or plastu00eddico, respectively.The cells in which the VSP is expressed. The invention further provides methods for producing plants with increased nitrogen content and / or increased nutritional value, which is desirable for commercial crops, including those used for forage, silage and grain production.In addition, the present invention provides a method for the production of a plant transformed with transformation of a cell of the plant with the construction of nucleotides, cultivation of the plant cell under conditions of growth and regeneration of transformed plant.