In-situ analysis the chemical composition of tibial articular cartilage of female Hartley guinea pigs with Fourier transform infrared (FTIR) microspectroscopy was conducted. The infrared spectrum survey consists of three ages (1 months, 2 months and 3 months)and three cartilage layers (surface, middle and deep). The results demonstrated that with ages increasing, the peak positions of main absorbance bands in surface and middle shifted to a lower wavenumber, and in deep they shifted to a lower wavenumber first, then shifted to a higher wavenumber. Infrared spectrum character of collagen, nucleic acid and proteo-glycan were compared and analyzed, The ratios of I1 657/I1 548 , I 1 074//I1 548 and I1 074/I1 237 tend to decrease with ages increasing in surface and middle. However, the ratios at 2 months are less than other ages in deep. These results are consistent with the regular pattern of cartilage ingredient change in different degradation stage, while the tibial platform images created by microscopic spectral imaging technology is highly compliant with pathology description. The authors' primary result illustrated that FTIR microspectroscopy can be used for in-situ analysis of molecular constituents of different levels cartilages. The molecular information obtained from the study is important for understanding the pathogenesis of cartilage diseases.%应用显微傅里叶变换红外光谱技术原位分析雌性Hartley豚鼠胫骨关节软骨的化学组成.红外光谱测定包含三种月龄(1月、2月和3月)和3个软骨层次(表层、中层和深层).结果表明,随着月龄的增加,表层和中层FTIR光谱的主要吸收峰峰位红移,深层吸收峰先红移后蓝移.软骨中胶原蛋白、核酸和蛋白多糖的红外光谱具有如下特征:峰强比I1657/I1548,I1074/I1548和I1074/I1237在软骨表层和中层随月龄递降,而在软骨深层2月龄的比值最低.结果符合关节软骨在不同退变阶段化学成分的变化规律,且显微光谱成像技术转化的各月龄胫骨平台二维图像高度符合相应的病理描述.初步研究显示,显微FTIR技术可原位分析不同层次软骨组织的分子组成,能为研究软骨疾病的发病机理提供可靠信息.
展开▼
