Using cyanine dyes supramolecular as molecular probes to mark FA has important significance in life sciences and pharmaceutical chemistry ,w hich can detect FA as drug efficacy mechanism and the change in physiological activity .In the pres-ent paper ,we investigated supramolecular assembly and chiral inducement of cyanine dyes template by FA with absorption and circular dichroism (CD) spectra .The result suggests that FA can induce cyanine dyes from J-aggregation to monomer along with different colors change and has strong affinity with cyanine dye monomer .The template of FA not only can translate the chirality of MTC H-aggregation to other two states ,but also induce ETC J-aggregation to molecular rearrangement and form left-handed helix of J-aggregates .Besides ,the association of PTC with FA ,i .e .binding to FA gave rise to the J-aggregation CD signals . Meanwhile ,it was inferred that the meso substituent of cyanine dyes play an important role in the interaction between FA and the J-aggregation:the smaller the meso substituent ,the higher the affinity interacted with FA .Clearly ,the binding abilities be-tween cyanine dyes and FA follow the order of MTC> ETC> PTC .These results support that the cyanine dyes supramolecular aggregates can be used as a kind of excellent molecular probes for specific recognition of FA and achieve the effect of visual in-spection .%利用菁染料超分子作为分子探针对黄腐酸进行标记，检测黄腐酸和药效机制，对于生命科学和药物化学领域的发展具有重要意义。采用紫外-可见吸收光谱和圆二色谱研究黄腐酸作为模板对不同结构菁染料超分子的组装及手性调控。结果表明：黄腐酸能诱导三种菁染料 J-聚集体解聚为单体并伴有体系表观颜色的变化，并与菁染料单体之间具有很高亲和力。黄腐酸作为模板不仅可以诱导M TC形成具有一定手性的H-聚集体，而且能使M TC的 H-聚集体的手性出现两次反转；还可以诱导ETC的J-聚集体发生分子间的重排，最终形成具有左手螺旋性的J-聚集体。除此之外，黄腐酸还对PTC的J-聚集体圆二色谱信号有显著的影响。同时，菁染料中位取代基影响到相互作用的能力：中位取代基越小，菁染料聚集体与黄腐酸的作用越强。菁染料与黄腐酸的结合能力大小为M TC＞ETC＞ PTC。菁染料超分子可作为一类优良的分子探针对黄腐酸进行特异性识别并可达到可视化的检测效果。
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