仙茅苷的超声辅助回流提取工艺优化

摘要

优选中华仙茅中仙茅苷的提取工艺。通过单因素试验和Box －Benhnken试验设计，确定仙茅苷超声辅助回流提取的最佳工艺参数为乙醇浓度80％、提取功率320W、料液比1∶10；通过高效液相色谱分析与响应面分析，得到的超声辅助回流法提取仙茅苷的分析预测提取率为161.26mg／100g ，通过实验验证得到实际值为160.82mg／100g。软件分析与实验提取工艺优化的结果相差极小，工艺优化参数较好。%Optimal Choosing Chinese Curculigo curculigoside extraction process .Through single factor and Box -Behnken experimental design to determine curculigoside ultrasonic assisted reflux extraction experiment's optimum parameters ,the ethanol concentration is 80% ,the extraction power is 320W ,solid-liquid ratio is 1∶10;By HPLC analysis and Response Surface analysis ,analysis of the resulting ultrasound -assisted re-flux extraction curculigoside extraction rate was 161.26mg/100g ,under the conditions of extracting power of 320W ,ethanol concentration was 80% ,solid-liquid ratio of 1∶10 ,experimental validation of the actual value was 160.82mg/100g .Software analysis and experimental results of extraction process optimization had minimal difference ,thus ,this process parameter optimization is suitable .