正交试验优选人参皂苷 Re 转化为 Rg 2发酵工艺

摘要

优选人参皂苷Re转化为人参皂苷Rg2最佳发酵工艺条件，计算人参皂苷Rg2转化产率。采用HPLC法，从发酵时间、发酵温度、复合菌添加量3方面，以人参皂苷Rg2转化产率为评价指标，优选人参发酵条件，并进行相关性分析。人参发酵工艺为酶解温度60℃、加酶量10％、发酵时间10d。采用正交实验优选人参发酵工艺条件，该实验可以为人参皂苷Rg2的工业化生产提供新的途径。%Fermentation technology optimization of ginsenoside Re biotransformed into ginsenoside Rg 2 by orthogonal test and calculate yield of ginsenoside Rg2 ;Useing fermation time ,fermation temperature ,added content of composite enzymatic as model by HPLC ,to optimization gin-senoside fermentation condition and correlation analysis ;Ginsen fermentation technology was fermation temperature 60℃ ,added content of com-posite enzymatic 10% ,fermation time 10 days;To apply the fermentation technology optimization of ginsenoside ,it is the new way available for the ginsenoside Rg2 industrialized production .