以小麦(扬麦5号)幼苗为材料，研究了铝对小麦幼苗根系生长、细胞膜脂质过氧化作用、质膜及液泡膜微囊ATPase活性的影响和外加钙缓解Al毒的缓解效应。结果显示，在Al3＋胁迫下，增加Ca2＋的供应可以减轻Al3＋对植物的毒害，明显减轻Al3＋处理对根系伸长及干物质积累的抑制作用，并可减轻因Al3＋处理诱导的根系电解质渗漏率和MDA含量的增加和过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性的抑制。根系质膜和液泡微囊的H＋- ATPase活性也因Al3＋处理而下降，增加Ca2＋的供应则可以提高H＋ -ATPase活性，降低H＋-ATPase对ATP的Km值和提高Vmax值%The effect of Al3＋ on the root growth, the li pid peroxidation, the H＋-ATPase activities of plasma and tonoplast and the m itigative effect of Ca2＋ were studied using wheat cv. Rangmai 5 as mate rial. It was shown that the root growth and dry matter accumulation were interfe red, the lipid peroxidatic reaction(MDA content) and electrolyte leakage of root s were increased, the activities of CAT, APX and SOD in roots and the H＋-ATP ase activities of plasma and tonoplast from root cells were decreased under Al 3＋ treatment. At the same time, the Vmax of two H＋-ATPases were decreased and the km were increased under Al3＋ treatment. The effects of Al3＋(under 0.5 mmol/L Ca2＋ level) were mitigated by increasing Ca2＋ supply from 0.5 to 5.0 mmol／L in nutrient solution.
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