中草药提取液对3种柑橘病原菌的抑制作用

摘要

为了对中草药提取物应用于水果保鲜领域和开发新型植物源农药提供理论基础和依据,在中药质量浓度为0.05g/mL条件下,测定19种中草药水提物对3种来自柑橘采后病害的病原菌意大利青霉(Penicillium italicum Wehmer)、指状青霉(Penicillium digitatum Sacc.)和柑橘链格孢(Alternaria citri Ellis et Pierce)的抑菌效果.结果表明:同一种中药对不同病原菌及不同中药对同一病原菌的抑制作用存在差异.19种中草药水提物中,黄连对3种病原菌的综合抑菌率最高,为98.37%,且黄连提取物对意大利青霉,指状青霉,柑橘链格孢的最小抑菌浓度(MIC)分别为:0.2,0.025,0.05g/mL.而白芥子对意大利青霉以及桃仁、茯苓和玄参对指状青霉的生长则具有不同程度的促进作用.%The inhibition effect of 19 extract liquids with 0. 05 g/mL concentration from Chineae herbal medicines on Penicillium italicum. Penicillium digitatum and Alternaria citri of citrua diseasea was determined to provide the theoretical basis for application of Chineae herbal medicine extract in fruit preaervation and development of new batanical pesticiden. The results showed that there was difference in inhibition effect of the same extract liquid against different pathogens and inhibition effect of different extract liquids against the same pathogen , the comprehenaive inhibition rate of Coptis chinensis extract liquid against three pathogens reached 98. 37 ％ , the minimal inhibitony concentra tion of Coptis chinensis extract liquid against Penicillium italicum , Penicillium digitatum and Alternaria citri was 0 . 2 , 0. 025 and 0. 05 g/mL respectively , Sinapis alba extract liquid could promote growth of Peniciltium italicum , and liquida extracted from Prunus persica , Poria cocos and Scrophularia ningpoensis could promote growth of Penicillium digitatum to varying degrees.