马铃薯叶片愈伤组织再生体系的建立

摘要

The regeneration system of eight potato cultivars was studied with leafs as explants. The results showed that the optical callus induction medium was MS +6-BA 3 mg/L + NAA 0.3 mg/L + GA3 10 mg/L for Yunshu 501 and MS+6-BA 3 mg/L + NAA 0.5 mg/L + GA3 10mg/L for Hui-2, and the induction rate reached to 100 % and 95.7 %, respectively. The optical shoot induction medium was found to be MS+6-BA 3mg/L + GA3 10 mg/L for Yunshu 501 and MS+6-BA 3 mg/L + NAA 0.02 mg/L + GA3 10 mg/L for Hui-2, and the regeneration frequency reached to 86.3 % and 65.4%, respectively. In contrast, no or very low callus regeneration was obtained from other cultivars, indicating that regeneration efficiency was strongly genotype-depended.%以8个马铃薯品种(系)为试材,进行叶片离体再生研究.结果表明,云薯501愈伤组织诱导的最佳培养基为MS +6-BA3mg/L+NAA0.3 mg/L+GA3 10 mg/L,愈伤诱导率为100％,诱导不定芽分化的优化培养基为MS +6-BA3 mg/L +GA310 mg/L,芽的分化率为86.3％；会-2的愈伤组织诱导的最佳培养基为MS +6-BA3 mg/L +NAA 0.5 mg/L +GA3 10 mg/L,愈伤诱导率为95.7％,诱导不定芽分化的优化培养基为MS +6-BA3 mg/L +NAA0.02 mg/L +GA3 10 mg/L,芽的分化率为65.4％.上述2个品种适宜用作马铃薯转基因实验的载体,其他6个马铃薯品种(系)的愈伤诱导率很低,有的品种(系)没有愈伤组织产生,不能作为马铃薯基因转化的载体.