重庆地区猪流行性腹泻病毒M基因的序列分析

摘要

[Objective] In this paper,the sequence features of M gene of porcine epidemic diarrhea virus (PEDV) in Chongqing municipality were studied.[Method]Three samples from different pig farms in Chongqing during 2014-2015 were collected.RT-PCR method was used for gene amplification,M genes of three positive samples were sequenced,their nucleotide sequences by DNA star software were analyzed and compared with strains from other areas,and finally their phylogenetic tree were constructed.[Result] M gene in the nucleotide sequences of three positive samples were 426 bp,encoding 142 amino acids.It was found that the nucleotide homology of M genes among the three samples was 98.7 ％-99.8 ％,and compared with classic stain CV777,the homology was between 97.1％ and 98.2 ％.[Conclusion]These findings indicated that the M gene is relatively conservative and could be a target of clinical diagnosis.%[目的]本文研究了重庆地区猪流行性腹泻病毒(Porcine Epidemic Diarrhea Virus,PEDV)M基因序列的特征.[方法]2015年期间采集了来自重庆地区3家规模猪场的病料,采用RT-PCR的方法对PEDV的M基因进行了扩增,测序后进行了分析,并与中国其他省份、美国、韩国的PEDV毒株进行同源性比较,完成了进化树的构建.[结果]3份病料中M基因片段长为426 bp,编码142个氨基酸.测序表明,3份毒株M基因同源性为98.7％～99.8％.与经典毒株CV777相比,核酸序列同源性为97.1％-98.2％.[结论]说明PDEV病毒M基因相对保守,可以作为临床检测的靶标.