芒果八氢番茄红素合成酶基因PSY的结构分析及功能预测

摘要

为研究芒果八氢番茄红素合成酶基因(PSY)的性质及结构功能.根据GenBank上登录的芒果及其它11种植物的PSY蛋白质氨基酸序列,利用生物信息学在线分析软件对其组成成分与理化性质、系统发育进化、导肽、跨膜结构域、疏水性/亲水性、二级结构、功能结构域以及三级结构进行预测和分析.结果表明,芒果与其它11种植物的PSY基因氨基酸序列的理化性质基本一致,在进化关系上划分为2类,主要表现为亲水性蛋白,α-螺旋、不规则卷曲和延伸连是其二级结构的主要结构元件,没有跨膜结构域,属于萜类合成酶C1超家族.本研究将为深入揭示PSY基因在植物花、果实和果皮以及叶片等器官颜色变化中所发挥的功能,开展其分子机理研究提供理论参考.%In order to study the properties and structure functions of PSY enzyme of mango,according to the nucleic acid sequences and amino acid sequences of Phytoene synthase (PSY) from Mangifera indica and other eleven species,several parameters of these sequences logined in GeneBank,including sequences composition,physicochemical properties,phylogenetic evolution,leader peptides,topological structures of transmembrane regions,hydrophobicity / hydrophilicity,the secondary structures,functional domains and the tertiary structures by bioinformatics tools were predicted and analyzed.The result showed that physicochemical properties of PSY in Mangifera indica and other plants were alike.Phylogenetic analysis showed that PSY amino acid sequence of the twelve plants was classified into two classes.In all these PSY protein did not have transmembrane topological structures,and the main was a hydrophilic protein,α-helix,extended strand and random coil were the major motifs of predicted secondary structure of PSY and belonged to Isoprenoid Biosyn C1 superfamily.This work would provide a theoretical reference for studies on color variance of different plant organs,such as flowers,fruits and leaves.