团头鲂性腺发育不同时期组织学观察及Kiss2/Kiss2r基因表达分析

摘要

Lots of studies have proved that Kiss gene and its receptor gene Kissr play an important role in the regulation of the gonadotropic axis,especially in the timing of puberty in vertebrates.We identified the gonad development of blunt snout bream (Megalobrama amblycephala) from stage Ⅰ to stage Ⅵ using tissue slice technology,detected the Kiss2 and Kiss2r gene transcription in the brain through quantitative real time PCR (qPCR),and collected pituitary and gonad tissues of the samples during different stages.The qPCR results show that Kiss2/Kiss2r play roles in the onset of puberty in M.amblycephala by increasing the expression of pituitary and gonad during ovary development in stage Ⅱ,and the expression of testis in stage Ⅲ.%Kiss基因(Kisspeptin)及其受体Kissr基因(Kiss receptor)组成的系统参与下丘脑-垂体-性腺轴功能的调节,在脊椎动物青春期启动的过程中发挥重要作用.该研究通过组织切片技术观察了团头鲂(Megalobrama amblycephala)卵巢和精巢的不同分期,进一步采用荧光定量PCR技术分析比较了团头鲂Kiss2和Kiss2r基因在雌、雄性腺不同时期对应的脑、垂体、性腺组织中的表达量.组织切片观察明确鉴别出团头鲂卵巢和精巢发育Ⅰ期至Ⅵ期的性腺组织结构,基因荧光定量PCR分析结果表明Kiss2/ Kiss2r系统主要是通过增加团头鲂卵巢发育Ⅱ期时脑和垂体的表达量以及Ⅲ期精巢的表达量来发挥生殖启动作用.