利用BiologTM微平板检测技术研究了烤烟连作对土壤微生物功能多样性的影响.结果表明,烤烟连作7年土壤微生物群落平均颜色变化率(AWCD)显著低于轮作各处理；土壤微生物功能多样性丰富度指数、Shannon多样性指数和Simpson指数连作处理均低于轮作处理,且差异达到显著水平(P＜0.05)；连作7年处理土壤微生物功能多样性明显低于连作2年处理.在土壤微生物碳源利用上,烤烟连作2年和7年处理土壤微生物对聚类化合物、碳水化合物、羧酸类、氨基酸类和其他化合物的利用显著低于正茬轮作处理,表明烤烟连作降低了土壤微生物的种群多样性和功能多样性,而且连作年限增加不利于土壤微生物的多样性.%Soil microbial diversity and community structure under continuous tobacco cropping were analyzed by using Biolog microplates. The results showed that the average well color development (AWCD) of soil microbe in 7a continuous tobacco cropping were lower obviously than in rotation; the indices of richness, Shannon-Wiener and Simpson's diversity of microbial metabolic diversity of continuous tobacco cropping were all significantly lower than those of maize-soybean-tobacco or soybean-maize-tobacco rotation; and the functional diversity index of soil microbial community under 7a continuous tobacco cropping was lower than under 2a. The utilization of carbon sources by soil microbes was lower in continuous cropping than in rotation. It suggested that continuous tobacco cropping reduced the diversity of soil microbial community structure and function.
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