胰腺癌中miRNAs、mRNAs基因表达谱分析

摘要

In this paper,we study miRNAs-mRNAs regulatory mechanism about pancreatic cancer.Application of bioinformatics method,we set up the miRNAs-mRNA disease network by the pancreatic cancer expression profile,and obtain five significance functional module by mining module of plugins .The larger degree nodes of miRNAs play a role HUB node in this network,while these are closely related to the initial and development of pancreatic cancer. whereafter use of clustering method to each module. the small degree nodes are gathered a collection,of these are more closely related to pancreatic cancer. The GO annotation of genes showed that pancreatic cancer was closely correlated to the metabolism function; moreover,many of same functional annotation appear in multiple modules,while many contrary of functional annotation appear in the single module. This suggests that there is a balance in its original regulatory mechanism.In this paper,achieved some synergy results about of miRNAs-mRNAs,these results may provide reference and help for future disease research on pancreatic cancer.%本文研究miRNAs、mRNAs在胰腺癌中的调控机制。通过对胰腺癌表达谱进行分析，基于生物信息学方法，建立miRNAs-mRNAs网络，通过模块挖掘获得5个功能显著性模块，网络中结点度较大的HUB节点miRNAs，这些结点与胰腺癌的发生、发展密切相关。对每个模块进行聚类分析，得到度数小却聚集的集合，这些基因也和胰腺癌密切相关。对这些模块进行功能注释，多个模块注释到代谢相关的功能上，表明胰腺癌与代谢密切相关；另外，多个模块中具有多个相同功能注释，多个调控相反的功能在同一模块当中出现。这表明在组织自身原来就存在着平衡调控机制。通过本文的研究得到miRNAs与mRNAs协同作用的一些结果，为今后更深入的研究胰腺癌提供指导和借鉴。