以沙培霞多丽幼苗为试材，研究了不同氮素形态对葡萄光合特性及碳水化合物积累的影响。结果表明：与全硝、全铵相比，硝铵比70/30时霞多丽叶片的叶绿素总量分别增加了25%、26%，净光合速率分别提高了12.6%、20%。硝铵比70/30时，叶片、新梢的淀粉含量较高，分别是全硝的1.6倍、2倍，全铵的1.75倍、1.3倍；根系中的可溶性糖含量比全铵处理增加了37.8%；而根系中的淀粉含量是以硝铵比50/50时较高，分别是全硝、全铵的1.4倍、1.6倍。硝铵比70/30有利霞多丽叶片叶绿素的增加，提高了净光合速率，混合氮形态处理（硝铵比70/30、50/50、30/70）提高了霞多丽根系和叶片中淀粉和可溶性糖含量，全铵处理有利于茎干中淀粉、可溶性糖含量的提高。%In order to explore the effects of different NO3--N/NH4+-N ratios (100/0, 70/30, 50/50, 30/70, 0/100) on photosynthetic characteristic and carbohydrate accumulation in the vine, a sandy culture experiment was conducted by Chardonnay seedlings. The results showed that Chlorophyll content in leaves is the maximum in the treatment which the ratio of NO3--N/NH4+-N was 70/30, and increased by 25% and 26% respectively compared with complete NO3--N and NH4+-N treatments. The photosynthetic parameters of Chardonnay treated with ratio of NO3--N/NH4+-N was 70/30 was higher, and net photosynthetic rate increased by 12.6% and 20% compared with that of NO3--N and NH4+-N treatment respectively. The starch and soluble sugar in roots and leaves of Chardonnay with mixed nitrogen treatments were relatively high, and the starch content in grape roots reached to the peak when the ratio of NO3--N/NH4+-N was 50/50, which was 1.4 and 1.6 times of those plants treated with complete NO3--N and NH4+-N respectively. The soluble sugar content of roots and leaves in treatment which ratio of NO3--N/NH4+-N was 70/30 increased by 37.8% and 108%compared with complete NH4+-N treatment respectively. The starch and soluble sugar content of grape treated with NH4+-N were 2.63 and 1.4 times of that treated with NO3--N.
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