目的 探讨银杏叶提取物(Egb761)对哮喘大鼠气道上皮细胞基质金属蛋白酶-9(romp-9)表达的影响及对气道炎症的干预作用.方法 将SD大鼠随机分为4组,即正常对照组(A组),哮喘模型组(B组),地塞米松组(C组),Egb761组(D组),建立大鼠哮喘模型,用免疫组化技术结合计算机图像分析系统,测定各组大鼠气道上皮细胞mmp-9表达的相对含量,同时检测支气管肺泡灌洗液(BALF)中嗜酸性粒细胞(EOS)计数及细胞分类计数.结果 ①mmp-9表达的相对含量,哮喘模型组明显高于正常对照组(P＜0.05);Egb761组明显低于哮喘模型组(P＜0.05)且与地塞米松组差异无统计学意义(P＞0.05);②Egb761组BALF中EOS的表达明显低于哮喘模型组(P＜0.05)且与地塞米松组差异无统计学意义(P＞0.05).结论 Egb761可以减少哮喘大鼠气道上皮细胞mmp-9的表达,同时对气道的炎症有明显的干预作用.%Objective To investigate the effects of ginkgo bioba extract( Egb761 ) on matrix metalloproteinase-9 (mmp-9)expression and evaluate the intervention effect on airway inflammation in asthmatic rats model. Methods 40 SD rats were randomly divided into 4 groups: normal control group( group A), asthmatic model group( group B), dexamethasone group( group C)and Egb761 group( group D) . The models of asthma were established by repeated inhalation of ovalbulium. The expression of MMP-9 in bronchial epithelium in these groups were determined using immunohistochemical technique and computer assisted image analysis system . The Eos amounts and differential leukocytes counts in bronchoalveolar lavage fluid(BALF) were counted. Results ①The expression of mmp-9 in the epithelial cells of bronchi was significantly higher in the asthmatic group than normal control group(P ＜ 0. 05) . The expression of mmp-9 in Egb761 group was much lower than that in asthmatic model group(P ＜0. 05 ). There were no statistical significant difference between dexamethasone group and Egb761 group( P ＞ 0. 05 ). ②The Eos amounts in BALF of Egb761 group was much lower than that in asthmatic model group( P ＜ 0. 05 ). There were no statistical significant difference between dexamethasone group and Egb761 group (P ＞ 0. 05 ). Conclusion Egb761 could inhibit the expression of mmp-9 and decrease airway inflammation in asthmatic rats.
展开▼
