RegⅣ在结肠癌中的表达及其与结肠癌发生发展的关系

摘要

目的 研究正常组织和结肠癌组织中RegⅣ蛋白的表达,探讨其与结肠癌的发生发展关系.方法 采用SABC免疫组织化学法分析102例结肠癌组织及50例正常结肠黏膜中RegⅣ表达,结合临床病理资料采用x2检验进行统计学分析,并做RegⅣ表达与结肠癌临床分期的回归分析,探讨RegⅣ与结肠癌的表达及发生发展的关系.结果 正常结肠粘膜中RegⅣ无表达,结肠癌组织中RegⅣ阳性表达率为34.31％.其中,G1、G2和G3期结肠癌阳性率分别为0.00％、22.22％和68.66％,病理分级间比较差异具有统计学意义(P＜0.05)；临床分期的阳性率分别为T0(0.00％)、T1(33.33％)、T2(44.44％)、T3 (52.38％)、T4 (71.42％),各期间比较差异有统计学意义(P＜0.05),两者回归方程为Y=5.955+15.83x,相关系数r=0.973.结论 RegⅣ在结肠癌的高表达与其病理分级及临床分期有密切的关系,可作为判断结肠癌分化程度的一个指标.%Objective To explore the expression of colon carcinoma and the development relationship between Reg Ⅳ and colon carcinoma. Methods Expression of Reg IV in colon carcinoma was determined in 102 cases of colon carcinoma tissues and 50 normal mucous membrane of colon cases with SABC immunohistochemical method and clinic-pathological data of patients were collected and used to make statistical analysis. And we do the regression analysis of clinical stage of colon cancer and Reg IV expression to find their relationship. Results The positive rate of Reg IV expression was 34. 31% in 102 colon carcinoma tissues, but no expression of Reg IV in the normal colon tissue. The percentages of the positive cases for Reg IV were 0. 00% , 22. 22% , 68. 66% in pathological grades from grade one to grade three respectively ( P ＜ 0. 05). The percentages of the positive cases for Reg IV clinic stages of Ta, were T0(0. 00% ), Tl (33. 33% ) ,T2(44.44% ) , T3(52. 38% ) , T4(71.42% ) respectively ( P ＜ 0. 05). The regression equation between clinical stage of colon cancer and Reg IV is Y =5.955 + 15. 83x, and the correlation coefficient r =0. 973. Conclusion High expression of Reg IV in colon carcinoma was positively related to the pathologic grade and the clinic stage of tumor, which might be a valuable maker for the character of colon carcinoma.