EDS-40玻璃化液冻存小鼠4细胞胚胎的研究

摘要

Objective To study the cryoprotective effect of the solution of EDS-40 on mouse 4 cell embryo . Methods First test the verifying effect of EDS-40 and EFS-40 solutions, Then random chose 4 cell embryos of female mice to vitrify in EFS-40 or EDS-40 solutions at 25℃ and immerse into liquid nitrogen. Control group embryos were placed in program freezing e-quipment. All samples were cryopreserved 3 months. The embryos were rewarmed rapidly in 25℃ water,and agitated gently ,then embryos were transferred into cultural medium and cultured. Then watch the survival rates and the merogenic rates. Results Both EDS-40 and EFS-40 solutions had vitrifying effects. The survival rates of EFS-40. EDS-40 and control group were 87. 8%、 93. 47% and 80. 77% respectively;the merogenic rates of EFS-4O、EDS-4O and control groups were79. 83% 、 86.53% and 69. 23% respectively. Conclusion The effect of EDS-40 solution is better than that of EFS-40.%目的 研究玻璃化液EDS-40对冻贮小鼠4细胞胚胎的效果.方法 ①EFS-40和EDS-40玻璃化溶液的玻璃化测试.②随机将4细胞小鼠胚胎,在25℃室温下分别加入EDS-40和EFS-40玻璃化溶液,玻璃化后装管并迅速投入液氮中.对照组置于程序冷冻仪中进行程序化冷冻.各组均冻存3个月后,在25℃的水浴中复温后,用培养液反复洗涤后移入培养孔板培养,观察胚胎存活率及卵裂率.结果 两种玻璃化溶液能达到玻璃化效果；EFS-40、EDS-40及对照组冷冻复温后的存活率分别87.8％、93.47％、80.77％；EFS-40、EDS-40及对照组冻胚的卵裂率分别:79.83％、86.53％、69.23％.结论 EDS-40玻璃化液冻贮小鼠4细胞胚胎的效果明显优于EFS-40,是一种理想的玻璃化液.