血管内皮生长因子VEGF单核苷酸多态性与同步放化疗后食管鳞癌患者预后关系的研究

摘要

目的：研究血管内皮生长因子( vascular endothelial growth factor ,VEGF)基因单核苷酸多态性( single nucle-otide polymorphism,SNP)与同步放化疗后食管鳞癌患者预后的相关性。方法经病理学确诊的食管鳞癌患者100例,采用同步放化疗方案。治疗前采集入组患者空腹外周血,采用基因芯片法检测VEGF rs3025039及rs833061的SNP。随机抽取10%的样本行基因测序验证此方法的准确性,比较不同基因型食管鳞癌患者同步放化疗后生存期的差异。结果VEGF rs3025039及rs833061两个位点的基因型频率分布均符合Hardy-Weinberg 平衡,且分布与年龄、性别并无关联。中位随访时间22个月,VEGF rs3025039 CT+CC基因型同步放化疗后食管鳞癌患者MST( Median survival time)、1年、2年和3年生存率为96．6%、71．4%、26．8%,均优于TT基因型患者60．0%、18．8%、9．42%,(P<0．05)；VEGF rs833061 CC基因型患者同步放化疗后2年生存率(51．3%)优于CT+TT基因型患者(21．7%)(P<0．05)。两组间MST、1年和3年生存率无统计学差异(P>0．05)。结论 VEGF rs3025039及rs833061基因多态性与ESCC患者同步放化疗后的生存期存在相关性,是预测ESCC患者同步放化疗后生存期的潜在指标。%Objective To investigate the association of the single nucleotide polymorphism( SNP) of vascular endothelial growth factor ( VEGF) gene and overall survival in locally advanced esophageal squamous cell carcinoma ( ESCC) patients after re-ceiving concurrent chemoradiotherapy ( CRT) . Methods A total of 100 patients with ESCC were routinely treated by CRT. The polymorphie genotypes of VEGF rs3025039 and rs833061 were analyzed by gene-chip method. DNA samples were isolated from pe-ripheral blood before CRT. Furthermore, we extracted 10% samples randomly for accuracy of this method. To explored the associa-tion between SNP of VEGF rs3025039, rs833061 and prognosis to CRT in ESCC patients. Results Genotype distributions had no deviation from Hardy-Weinberg equilibrium. Median follow-up was 22 months. Median survival time(MST), 1-year survival, 2-year survival and 3-year survival rates of ESCC patients with the VEGF rs3025039 CT +CC genotype and TT genotype were 26. 92 months, 96. 6%, 71. 4% and 26. 8% VS. 16. 54 months, 60. 0%, 18. 8% and 9. 42% (P<0. 05), respectively. Total-ly, 2-year survival of VEGF rs833061 CC genotype and CT+TT genotype were 51. 3% VS. 21. 7% (P<0. 05), respectively. MST,1-year survival, and 3-year survival rates have no difference(P>0. 05). Conclusion Polymorphisms of VEGF rs3025039 and rs833061 might be associated with overall survival in ESCC patients after CRT, and they might be the predictive bio-markers for overall survival.