De novo identification of mammalian ciliary motility proteins using cryo-EM

摘要

Dynein-decorated doublet microtubules(DMTs)are critical components of the oscillatory molecular machine of cilia,the axoneme,and have luminal surfaces patterned periodically by microtubule inner proteins(MIPs).Here we present an atomic model of the 48-nm repeat of a mammalian DMT,derived from a cryoelectron microscopy(cryo-EM)map of the complex isolated from bovine respiratory cilia.