自制5’-核苷酸酶试剂在生化检验中的应用

摘要

Objective:To evaluate the application of the independent innovation reagent in the automatic determination of 5’-nucleotidase (5’-NT) activity in the clinical serum or plasma samples.Methods: 5’-NT activities were determined with Olympus AU680 automatic biochemical analyzer by repeatability and linearity tests, the evaluation of methodology was carried out and the results were compared with those determined by the reagent of Diazyme Laboratories.Results: The coefficients of variation (CV) of the enzyme activity at two levels of quality control were 2.83% and 1.96%. The determination of enzyme activity showed a good linearity over the range of 5~200 U/L. There was a good correlation between two reagents (r=0.995). Conclusion:The 5’-nucleotidase reagent made by our company can provide a simple, quick, accurate and stable method for the determination of 5′-NT activity in the clinical serum or plasma samples and meet the requirement of the automated analysis of mass clinical samples. This method may be worthy of promotion.%目的：评价自主研发的5’-核苷酸酶试剂（速率法）在自动化检测血清或血浆中5’-核苷酸酶活性的应用。方法：采用奥林巴斯AU680全自动生化分析仪，通过重复性、线性试验、测定血清或血浆中5’-核苷酸酶活性，进行方法学评价并与美国Diazyme 5’-核苷酸酶试剂（速率法）比较。结果：两个水平质控中酶活性的变异系数（CV）分别为2.83%和1.96%，在5～200 U/L酶活范围内，线性良好。两种试剂测定血清5’-核苷酸酶活性的相关系数较好，相关系数r为0.995。结论：自主研发的5’-核苷酸酶试剂盒操作简便、快速、准确、稳定，结果准确，可满足大批量临床样本的常规和自动化分析，是一种值得推广的方法。