Objective To investigate the regulating effect of fire needling on Wnt andβ-catenin genes of Wnt/β-catenin pathway in neural stem cells in spinal cord injury.Methods Sixty female SD rats were randomly allocated, including 5 rats to a blank group, 5 rats to a sham operation group, 25 rats to a model group and 25 rats to a fire needling group. The model and fire needling groups of rats were again separately divided into 1 d, 3 d, 7 d, 10 d and 14 d groups. A model was made using modified Allen's method in the model and fire needling groups. The BBB score was recorded and the expressions of Wnt andβ-catenin genes were determined in every group.Results In the model and fire needling groups, the BBB scores were significantly higher at 7 and 10 days than at the previous time point and there was a statistically significant difference (P<0.05). The BBB score at every time point after treatment was significantly higher in the fire needling group than in the model group and there was a statistically significant difference (P<0.05). Wnt3a gene expressions increased in the model and fire needling groups at 7 and 10 days compare with the previous time point (P<0.05). At 7 and 10 days, Wnt3a gene expression was higher in the fire needling group than in the model group and there was a statistically significant difference (P<0.05). The changing tendency ofβ-catenin gene expression levels was basically the same as that of Wnt3a's.Conclusion Fire needling can modulate the expressions of Wnt3a andβ-catenin genes in neural stem cells in spinal cord injury.%目的 观察火针对脊髓损伤Wnt/β-catenin通路上神经干细胞Wnt、β-catenin基因的调控作用.方法 将60只SD雌性大鼠随机分为空白组5只、假手术组5只、模型组25只、火针组25只,模型组和火针组大鼠又分为1 d组、3 d组、7 d组、10 d组和14 d组;采用改良的Allen's法对模型组和火针组造模,观察各组BBB评分,Wnt3a和β-catenin基因表达变化.结果 在7 d、10 d时模型组和火针组BBB评分明显高于同组前一时相,差异具有统计学意义(P<0.05);火针组大鼠在治疗后各时间点BBB评分明显高于模型组,差异具有统计学意义(P<0.05).在7 d、10 d时模型组和火针组Wnt3a基因表达量较同组前一时相均增加(P<0.05);在7 d、10 d时火针组Wnt3a基因表达量高于模型组,差异具有统计学意义(P<0.05);β-catenin基因表达水平变化趋势与Wnt3a基本一致.结论 火针可调控脊髓损伤神经干细胞Wnt3a、β-catenin基因表达.
展开▼
