棉花黄萎病生防细菌NCD-2抑菌物质提取研究

摘要

应用微生物控制植物病害是非常有效的方法.枯草芽孢杆菌是最著名的产生抗生作用的拮抗细菌.本文所使用的防治棉花黄萎病生防细菌NCD-2是枯草芽孢杆菌菌株,对16种植物病原真菌具有抑制作用,并且对棉花黄萎病具有较高的田间防病效果.通过发酵培养、有机溶剂萃取和盐析和透析提取了该菌株的胞外分泌产物,经抑菌作用测定证明该菌株分泌的有效抑菌成分为耐热蛋白.%The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens.There are many examples of formulations using bacterial or fungal strains with biocontrol applications. Among them, members of the genus Bacillus are well known antibiotic producers.This research refers to NCD-2 bacillus subtilis strain, an antagonist invitro against 16 plant pathogens and a paramount and potential biocontrol agent with high efficacy of control on verticillium wilt of cotton in greenhouse and in field, in order to determine its extracellular metabolites with antibiotic activity and the antibiotic properties.The results revealed the antibiotic metabolite maybe a thermostable protein.