We constructed a HPLC based content determination method for emodin and emodin monomenthyl ether from Shouwuyizhi capsule. Samples were separated by C18(250 nm×4.6 nm, 5μm) column with methanol-0.1%phosphoric acid water ( 82:18, V/V) as mobile phase. Determination conditions were determination wavelength of 289 nm, flow rate of 1.0 mL/min, and column temperature of 30℃. Better linear relationship exists in the range 0.019~0.19μg( R=0.9999,n=5) for emodin, and in the range 0.021~0.21μg( R=1.0000,n=5) for emodin monomenthyl ether. Their average recovery rates are respectively 98.58%and 100.01%, and RSDs are 2.66%and 1.15%. Results show that the method has strong specificity and accuracy, so it can be applied to simultaneous content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule.%建立了高效液相色谱法(HPLC)同时测定首乌益智胶囊中大黄素和大黄素甲醚含量的方法.采用C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸水(82:18,V/V)为流动相,检测波长为289 nm,流速为1.0 mL/min,柱温30℃.测得大黄素在0.019~0.19μg(R=0.9999,n=5)、大黄素甲醚在0.021~0.21μg(R=1.0000,n=5)线性关系良好;大黄素和大黄素甲醚的平均回收率分别为98.58%、100.01%,相对标准偏差分别为2.66%、1.15%.研究表明,该方法专属性强、结果准确,可作为同时测定首乌益智胶囊中大黄素和大黄素甲醚含量的方法.
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