酶电极法测定玉米中黄曲霉毒素B1含量

摘要

黄曲霉毒素与其氧化酶接触时会发生氧化还原反应,产生过氧化氢及其他产物.本研究采用酶电极生物传感器,将黄曲霉毒素氧化酶固定在醋酸纤维素载体膜上,制备电流型电化学酶电极.在SBA流动注射分析仪器上安装黄曲霉毒素氧化酶电极,检测黄曲霉毒素含量.结果表明,黄曲霉毒素酶电极对黄曲霉毒素具有良好的响应特征,测定精密度(RSD)为1.20％ (n=10),线性范围为0～100 μg/L(R2=0.999 6),加标回收率为96％～ 102.4％.因此,本研究建立的酶电极分析法可用于玉米中黄曲霉毒素B1的检测.%The redox reaction will occur when aflatoxin contacts with its oxidase,which can produce hydrogen peroxide and other products.In this research,the method of enzyme electrode biosensors was used to immobilize aflatoxin oxidase on cellulose acetate carrier membrane and prepare electrochemical enzyme electrode.Aflatoxin oxidase electrode was installed on the SBA flow injection analysis instrument to detect the content of aflatoxin.The results showed that the toxin enzyme electrode had good response characteristics to aflatoxin,the precision (RSD) was up to 1.20％ (n =10),the linear range was 0 ～ 100 μg/L (R2 =0.999 6),and the recovery rate was 96％ ～ 102.4％.Therefore,the enzyme electrode analysis method established in this study can be used for the detection of aflatoxin B1 in maize.