参附注射液对人慢性髓性白血病细胞来源的树突状细胞诱导生成的影响

摘要

Objective To investigate the effects of shenfu injection (SFI) on the induction of chronic myeloid leuke mia cell line K-562 cells into dendritic cells (DCs). Method In vitro, K-562 cells were cultured with different concen trations SFI, granulocyte-macrophage colony-stimulating factor ( GM-CSF)/interleukin-4 (IL-4) and GM-CSF/IL-4 com bined with different concentrations of SFI, respectively. Results In vitro, DCs could not be derived from K-562 cells cul tured with different concentrations of SFI. While DCs derived from K-562 cells co-cultured with GM-CSF/IL-4 could ex press phenotype of antigen-presenting cells and have the similar immune function with normal DCs, but the surface markers and the function were unmature. However, different concentration of SFI plus GM-CSF/IL-4 could not only induce matura tion of K-562 DCs but inhibit propagation of cells. Cells cultured with middle concentration SFI (75 mg/ml) plus GM-CSF/IL-4 expressed higher levels of phenotypes, elicited significantly stronger abilities of stimulating T lymphocyte proliferation, and secreted larger amounts of IL-12. Conclusions In vitro, appropriate concentrations of SFI plus GM-CSF/IL-4 can induce differentiation and maturation of K-562 DCs, change the expression of their surface markers and enchance their immune stimulatory function. The best concentration of SFI is 75 mg/ml which plus GM-CSF/IL-4 can induce K-562 cells into DCs.%目的 研究参附注射液(SFI)对慢性髓性白血病细胞系K-562细胞诱导生成树突状细胞(DCs)的影响.方法 用不同浓度的SFI、粒一巨噬细胞集落因子(GM -CSF)/白细胞介素4(IL-4)及GM-CSF/IL-4联合不同浓度SFI 3种组合在体外诱导培养K-562细胞.结果 体外单用SFI不能诱导K-562细胞分化为DCs.单用GM-CSF/IL-4可诱导K-562细胞分化为DCs,并具有DCs的特异性标志及功能,但不成熟.不同浓度的SFI联合GM-CSF/IL-4对K-562 DCs有促进成熟和抑制增殖的双向调节作用,其最佳诱导浓度为75 mg/ml.结论 适当浓度的SFI联合GM-CSF/IL-4在体外能更有效地诱导K-562细胞分化为DCs,并促进DCs成熟,增强DCs功能,且75 mg/mlSFI与GM-CSF/IL-4联合有协同作用.