Objective To investigate the effects of matrix metalloproteinase 1 ( MMP-1) and tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) in congenital heart disease (CHD) with pulmonary hypertension (PH). Methods Sixty patients with CHD were observed of pulmonary artery morphology and divided into 4 groups according to Heath-Edwards pulmonary vessel pathologic grading; control group (without PH, n = 14) and PH Ⅰ ,Ⅱ and Ⅲ combined with CHD groups ( n = 46). RT-PCR and immunohistochemical SP method were employed to detect the Mrna and proteins of MMP-1, TIMP-1 in the lung tissues of these patients. Results There were differences in the expression of MMP-1 Mrna, TIMP-1 Mrna, TIMP-l/MMP-1, proteins of MMP-1 and TIMP-1 in control group and experimental groups. There was significantly positive correlation between above-mentioned index and the grade of PH. Conclusions Increased levels and imbalance of MMP-l/TIMP-1 associate with the development and acceleration of PH in CHD. They may play a role in the pathogenesis of pulmonary vascular remodeling.%目的 探讨基质金属蛋白酶1( MMP-1)及其抑制因子1(TIMP-1)在先天性心脏病(CHD)合并肺动脉高压(PH)发病机制中的可能作用.方法 光镜下观察60例CHD患者肺小动脉形态,按照Heath-Edwards PH分级法分组,CHD无PH患者14例为对照组,CHD合并PHⅠ、Ⅱ、Ⅲ级共46例为实验组.采用免疫组化SP法和RT-PCR法分别测定患者肺组织MMP-1TIMP-1蛋白及mRNA的表达情况.结果 MMP-1 mRNA、TIMP-1 mRNA、TIMP-1/MMP-1、MMP-1蛋白、TIMP-1蛋白在对照组及各实验组间存在差异,且均与PH病理分级正相关(P＜0.05或＜0.01).结论 MMP-1、TIMP-1表达增高和二者比例失衡与CHD并发PH的形成与发展有关,可能是PH患者肺血管重构的发病机制之一.
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