Objective To explore the change of NADPH oxidese and the intervention effect of atorrsatin on NADPH oxidase of atorvsatin aortic smooth muscle in rats after myocardial infarction ( MI). Methods Male Sprague-Dawley rats were divided into operated group and sham group according to ligation or not of anterior descending coronary artery. The operated rats were assigned randomly to intragastrically receive atorvastatin[ 10 mg/(kg · day) ] (MI intervention group)or saline(MI group) respectively on the second day after operation for 5 weeks. On the sixth week, the rats were killed and aortic smooth muscle was taken. The concentration of superoxide(O2- ) was measured with WST-1 method and the expressions of Noxl mRNA and Nox4 mRNA were detected with RT-PCR, and the correlations between the concentration of O2 and the expression of Noxl mRNA and Nox4 mRNA were respectively calculated. Results The concentration of O2- and the expression of Noxl mRNA and Nox4 mRNA in MI group increased significantly compared to sham group (P < 0.01) and MI intervention group ( P < 0.05 ). The concentration of O2- positively correlated to the expression of Noxl mRNA and Nox4 mRNA (r=0.53, P <0.05;r=0. 56, P<0.05 respectively). Conclusions The NADPH oxidase system is activated in aortic smooth muscle of rats after MI. Atorvsatin decreases the production of O2- by inhibiting the expression of Noxl mRNA and Nox4 mRNA.%目的 探讨心肌梗死后大鼠主动脉平滑肌NADPH氧化酶的变化及阿托伐他汀的干预作用.方法 取雄性SD大鼠,通过是否结扎左前降支分为手术组和假手术组,手术组大鼠于术后第二天随机给予阿托伐他汀[10mg/(kg·d)](心梗干预组)或等体积生理盐水(心梗组)灌胃共5周,第6周处死大鼠取胸主动脉平滑肌,采用WST-1法及RT-PCR法分别测定平滑肌超氧阴离子(O-2)浓度及Nox1 mRNA和Nox4 mRNA表达,并探讨其相关性.结果 心梗组O2-浓度、Nox1 mRNA及Nox4 mRNA表达高于假手术组(P＜0.01)和心梗干预组(P＜0.05).O2-浓度分别与Nox1 mRNA及Nox4 mRNA表达呈正相关(r=0.53,P＜0.05；r =0.56,P＜0.05).结论 心梗后大鼠平滑肌NADPH氧化酶系统被激活,阿托伐他汀通过降低Nox1 mRNA及Nox4 mRNA表达而减少O2-产生.
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