宫颈上皮内瘤样病变及宫颈癌组织中JNK、STAT3蛋白的表达变化及意义

摘要

Objective To observe the expression changes of c-Jun N-terminal kinase(JNK) and signal transducer and activator of transcription 3( STAT3) in cervical intraepithelial neoplasia( CIN) and cervical cancer( CC) tissues and to explore the significance. Methods The JNK and STAT3 protein expression was detected by immunohistochemical SABC method in 10 cases of normal cervical epithelium( NCE) , 105 cases of cervical intraepithelial neoplasia( CIN) and 66 cases of cervical cancer( CC) , and then the relationship of JNK and STAT3 with clinicopathological parameters of cervical cancer was analyzed. Results The positive expression rate of JNK protein in NCE, CIN and CC tissue was 10.0% , 45. 7% and 75.8% respectively. The differences above all have statistic significance ( P <0. 05). Meanwhile, the positive expression rate of STAT3 protein in NCE , CIN and CC tissue was 10% ,48.6% and 83.3% respectively. The differences above all had statistic significance(P <0. 05). The JNK and STAT3 expression was not related to age, clinical stage and lymph node metastasis, but related to histological grade of CC. The expression higher in poorly differentiated carcinoma than in well-moderately differentiated one( P < 0.005, x2 = 12. 08,12.61). Conclusions The expression of JNK and STAT3 protein in cervical intraepithelial neoplasia and cervical cancer increase and correlate with the carcinogenesis, development and differentiation of cervical cancer. JNK and STAT3 may be promising candidates for predicting prognosis in CC and have important implications for individual treatment.%目的 观察宫颈上皮内瘤样病变(CIN)及宫颈癌(CC)组织中c-Jun氨基末端激酶(JNK)和信号转导及转录活化因子3(STAT3)的表达变化,并探讨其意义.方法 采用免疫组化链霉亲和素—生物素—过氧化物酶(SABC)法检测10例正常宫颈组织(NCE)及105例CIN、66例宫颈癌(CC)组织中JNK和STAT3蛋白的表达情况,并分析其阳性表达率与CC临床病理参数的关系.结果 JNK蛋白在NCE、CIN及CC组织中的阳性表达率分别为10.0％、45.7％、75.8％,三者两两相比,P均＜0.05;STAT3蛋白在NCE、CIN及CC组织中阳性表达率分别为10.0％、48.6％、83.3％,三者两两相比,P均＜0.05.JNK、STAT3蛋白阳性表达均与CC患者年龄、临床分期、淋巴结转移无关,但与肿瘤组织学分级有关,其中低分化癌组织JNK、STAT3蛋白表达显著高于高、中分化癌组织(P＜0.005,x2=12.08、12.61).结论 JNK、STAT3蛋白在CIN及CC组织中的表达增加,与CC发生、发展及分化有关；检测两者表达可作为判断CC患者预后不良的客观指标及指导个体化治疗的依据.