原发性高血压患者子代人群载脂蛋白E基因多态性与高血压的关系

摘要

Objective To investigate the relationship between polymorphisms of apolipoprotein E ( ApoE) gene and hypertension of essential hypertension filial generation. Methods ApoE gene polymorphism was genotyped in 60 cases with essential hypertension filial generation (case group) and 60 normal cases (control group) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the relationship between polymorphisms of ApoE gene and hypertension was analyzed. Results All the research object of PCR fragments were satisfied with amplification, a total of 6 kinds of of genetic type were detected, the Hardy-Weinberg balance test was achieved linkage equilibrium, with group representative. In the two groups, ApoE3/3 was the dominant type, but there was no statistical difference in the all genotypes. In the two groups, e3 allele frequency was the highest and e2 was lowest. The e4 allele frequency was higher in case group than that in control group(x2 =12.42, P <0.05). Conclusions The ApoE e4 allele may be a hypertension susceptibity gene, the occurrence of hypertension might be correlative with a variety of factors, such as environment.%目的 探讨原发性高血压患者子代人群载脂蛋白E(ApoE)基因多态性与高血压的关系.方法 应用聚合酶链反应—限制性片段长度多态性技术检测60例血压正常的原发性高血压患者子代(研究组)和60例正常对照者(对照组)的ApoE基因多态性,并分析其多态性与高血压的关系.结果 所有研究对象的PCR片段均得到满意扩增,共检测出6种基因型,经Hardy-Weinberg平衡检验均达到连锁平衡,具有群体代表性.两组均以ApoE3/3为优势基因型,但两组各基因型比较差异均无统计学意义(P均＞0.05).两组均以ε3等位基因频率最高,ε2等位基因最低；研究组ε4等位基因频率高于对照组(x2=12.42,P＜0.05).结论 原发性高血压患者子代人群高血压的易感基因为ApoEε4等位基因.