青岛地区汉族老年原发性高血压患者载脂蛋白A5基因多态性研究

摘要

目的 研究青岛地区汉族人群中载脂蛋白A5基因(APOA5)-1131T/C多态性与老年原发性高血压病之间的关系.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测130例老年原发性高血压病患者(老年高血压组)和120例健康体检者(对照组)APOA5-1131T/C多态性基因型和等位基因频率分布.同时收集2组研究对象的体质量指数(BMI)、血压水平和生活方式,采用生物化学方法检测研究对象的血脂、脂蛋白和血糖水平.结果 老年高血压组BMI、吸烟史、高血压病家族史、收缩压(SBP)、舒张压(DBP)、总胆固醇(TC)、甘油三酯(TG)及低密度脂蛋白胆固醇(LDL-C)均高于对照组,高密度脂蛋白胆固醇(HDL-C)水平低于对照组(P<0.01).APOA5-1131T/C多态性PCR扩增片段大小为188 bp.老年高血压组中APOA5-1131T/C位点基因型以TC型多见,其次为TT型,而CC型比较少见;对照组中APOA5-1131T/C位点基因型以TT型多见,其次为TC型,而CC型比较少见;2组等位基因频率分布均以T等位基因为常见等位基因,C等位基因为少见等位基因.2 组间基因型频率和等位基因频率分布差别均有统计学意义(P<0.05,P<0.01).APOA5基因型频率的相对风险分析显示,CC型纯合子和TC型杂合子患高血压病的风险高于TT型纯合子(P<0.05).2组APOA5-1131T/C多态性中TC和CC基因型的TG水平高于TT基因型(P<0.01),其他血脂指标各基因型间差别无统计学意义(P>0.05).结论 APOA5-1131T/C多态性影响血浆TG水平,APOA5-1131C等位基因可能是青岛地区汉族人老年高血压发病的独立危险因素.%Objective To investigate the association beNeen -1131T/C polymorphism in apolipoprotein A5 ( APOA5 ) rngene and the aged patient with essential hypertension in Han people in Qingdao region. Methods The genotypes and allele rnfrequency distribution of APOA5-1131T/C gene polymorphism in 130 aged patients with essential hypertension ( aged hyperrntensive group) and 120 cases of healthy persons ( control group ) were analyzed by polymerase chain reaction-restriction fragrnment length polymorphism ( PCR-RFLP) method. The body mass index ( BMI) ,level of blood pressure and life style were colrnlected in two groups , and the levels of blood fat,lipoprotein and blood sugar were detected by method of biochemistry. Results rnSmoking history,family history of hypertension, the levels of BMI,systolic blood pressure ( SBP) , diastole blood pressure rn( DBP) , total cholesterol( TC) , triglycercide ( TG) and low density lipoprotein-cholesterol( LDL-C) in aged hypertensive group rnwere higher than those in control group, the level of high density lipoprotein-cholesterol( HDL-C) was lower than that in control rngroup ( P ＜0.01) . PCR amplified fragment of APOA5-1131T/C gene polymorphism was 188 bp. The genotypes distribution of rnAPOA5-1131T/C gene polymorphism were TC-type locus in common, followed by TT-type.and CC-type was relatively rare in rnaged hypertensive group,and TT-type locus in common,followed by TC-type,and CC-type was relatively rare in control group.rnT allele was a common allele and C allele was a rare allele in the allele frequency distribution in two groups. There were statisrntically significarU differencea in genotype and allele frequency distribution in two groups ( P ＜ 0. 05 , P ＜ 0. 01 ) . Relative risk arnnalysis of APOA5 genotype frequency showed the risk of suffer from hypertension for CC-type homozygous and TC-type heterornzygous higher than that in TT homozygous ( P ＜ 0. 05) . The levels of TG in TC-type and CC-type of APOA5-1131T/C gene polrnymorphism were higher than those in TT-type( P ＜0.01 ) . There was no statistically differences in other lipid parameters among rnthe Senotypes (P ＞0. 05). Conclusion The APOA5-1131T/C gene polymorphism might affecL the level of plaama TC,and rnAPOA5-1131C allele may be independent risk factor for the aged patient with essential hypertension in Han people in Qingdao rnregion.