胃癌组织中c-FLIP、NF-κB p65的表达变化及意义

摘要

目的 探讨细胞型Fas相关的死亡区域蛋白样白介素-1转换酶抑制蛋白(c-FLIP)、细胞核因子κB(NF-κB)在胃癌发生、发展中的作用.方法 选择胃癌组织标本80份(胃癌组)和癌旁组织80份(癌旁组),采用免疫组化法检测两组的c-FLIP、NF-κB p65,分析其与胃癌临床病理特征的关系.结果 胃癌组c-FLIP、NF-κB p65阳性率明显高于癌旁组(P均＜0.05)；c-FLIP的表达与胃癌临床分期、分化程度、淋巴结转移、术后复发相关(P均＜0.05),NF-κB p65的表达与浸润深度、分化程度、淋巴结转移、术后复发相关(P均＜0.05)；c-FLIP和NF-κBp65在胃癌组织中的表达呈正相关(r =0.303,P=0.006).结论 c-FLIP、NF-κB p65的高表达可能在胃癌的发生、发展中起重要作用.%Objective To investigate the roles of cellular Fas-associated death domain-like interleulin-1 0 converting engyme inhibitory protein (c-FLIP) and nuclear factor kappa B(NF-κB) p65 in the incidence and development of gastric carcinoma. Methods Collected 80 cases of gastric carcinoma tissue ( gastric carcinoma group) and 80 cases of paired adjacent gastric tissue( adjacent gastric carcinoma group) , immunohistochemisty was employed to determine the expression of c-FLIP and NF-κB p65 in the two groups. The relationship between c-FLIP and NF-κB p65, and their correlation with clinical parameters was analyzed. Results The positive rate of c-FLIP and NF- κB p65 was significantly higher in gastric carcinoma group than in adjacent gastric carcinoma group ( all P < 0.05). The expression of c-FLJP was significantly correlated with the clinical stage, the degree of tumor differentiation, the lymph node metastasis and the recurrence of tumor (all P < 0.05 ). The expression of NF-kB p65 was significantly correlated with the invaded depth, the degree of tumor differentiation , the lymph node metastasis and the recurrence of tumor (all P < 0.05 ) . There was a significantly positive relationship of c-FLIP with NF-κB p65 ( r = 0. 303 , P = 0. 006 ) . Conclusion The overexpression of c-FLIP and NF-kB p65 might play important roles in the incidence and development of gastric carcinoma.