目的：观察人参皂苷Rb1对H2 O2损伤RGC-5细胞的保护作用，并探讨其机制。方法采用MTT方法检测人参皂苷Rb1对H2 O2损伤RGC-5的细胞活力的影响，试剂盒方法检测SOD活性、GSH含量，免疫荧光化学染色法检测碘化丙啶（PI）、Caspase-3、Ca2＋。结果 A、B、C、D、E组在490 nm处检测细胞活力分别为0．266±0．038、0．299±0．025、0．314±0．014、0．222±0．031、0．391±0．041，A、B、C、E组与D组比较，P均＜0．05。空白对照组、人参皂苷Rb1组、H2 O2损伤组SOD活性分别为19．88±0．12、17．12±0．32、15．47±0．21，人参皂苷Rb1组、空白对照组分别与H2 O2损伤组比较，P均＜0．05。空白对照组、人参皂苷Rb1组、H2 O2损伤组GSH含量分别为15．67±0．18、14．98±0．25、13．71±0．31，人参皂苷Rb1组、空白对照组分别与H2 O2损伤组比较，P均＜0．05。空白对照组、人参皂苷Rb1组、H2O2损伤组Caspase-3阳性率分别为12％±0．25％、57％±0．47％、28％±0．18％，PI染色阳性率分别为16％±0．25％、18％±0．45％、32％±0．56％，Ca2＋荧光染色阳性率分别为8％±0．11％，31％±1．20％，19％±0．34％，人参皂苷Rb1组与H2 O2损伤组比较，P均＜0．05。结论人参皂苷Rb1能够拮抗H2 O2对RGC-5细胞的氧化损伤，其机制可能为提高SOD活性、GSH含量，降低Caspase-3表达，减少Ca2＋水平。%Objective To establish RGC-5 cell damage model induced by H 2 O2 , and to observe the protective effect of different dose of ginsenoside Rb 1 on RGC-5 apoptosis induced by H 2 O2 and find the possible mechanisms of the neuro-protection .Methods MTT was used to detect the effect of different dose of ginsenoside Rb 1 on RGC-5 activity induced by H2 O2 .SOD and GSH were detected by Kit method , and PI, Caspase-3, Ca2+were detected by immunofluorescence chemi-cal dyeing method.Results A, B, C, D, E groups at 490 nm for detection of cell viability were 0.266 ±0.038, 0.299 ±0.025, 0.314 ±0.014, 0.222 ±0.031, 0.391 ±0.041, A, B, C, E group compared with D group, all P<0.05.The control group, the experimental group, model group, SOD content were 19.88 ±0.12, 17.12 ±0.32, 15.47 ±0.21, and experimental group , control group were compared with the model group , all P<0.05.Experimental group and control group, model group, GSH content were 15.67 ±0.18 and 14.98 ±0.25, 13.71 ±0.31, and the experimental group and the control group were compared with the model group , all P<0.05.Compared with the model group , the experimental groups decreased Caspase-3 expression, suggesting that the 0.1 mM of ginsenoside Rb1 can significantly inhibit the expres-sion of Caspase-3.PI staining cells increased significantly in the model group , RGC-5 was obviously injured by H 2 O2 which lead to cell death .Apoptosis of cells were decreased in experimental group .Ca2+increased obviously in model group . Ca2+were significantly decreased in experimental group .Conclusion Ginsenosides Rb1 can prevent RGC-5 death in-duced by H2 O2 .The mechanism may be related to up-regulate SOD, GSH activity and reduce the activity of apoptosis-re-lated proteins Caspase-3 and Ca2+.
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