CCL3和 CCL4基因多态性与肺结核易感性的关系

摘要

Objective To explore the relationship of CCL3-2021C/T and CCL4-5725A/C gene polymorphisms with susceptibility to tuberculosis ( TB) .Methods 78 TB patients and 97 healthy controls were included in the study.CCL3-2021C/T and CCL4-5725A/C were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in two groups.Results CC, CT and TT genotype frequency of CCL3-2021C/T were 2.5%, 15.4%, 82.1% in TB group and 0, 18.6%, 81.4%in control group (all P>0.05).C allele frequency was 10.2%and T allele frequency was 89.8%in TB group;which were 9.3%and 90.7%in control group (all P>0.05).AA, AC and CC gen-otype frequency of CCL4-5725A/C were 7.7%, 30.8%, 61.5% in TB group and 5.2%, 27.8%, 67.0% in control group (all P>0.05).A allele frequency was 23.1%and C allele frequency was 76.9%in TB group, which were 19.1%and 80.9%in control group (all P>0.05).Conclusion Single nucleotide polymorphism (SNP) of CCL3-2021C/T and CCL4-5725A/C maybe not related to the susceptibility of TB infection.%目的：探讨趋化因子CCL3基因位点2021C/T和CCL4基因位点5725A/C的多态性与肺结核（ TB）易感性的关系。方法选择78例TB患者（结核组）和97例体检健康者（对照组），应用聚合酶链反应—限制性片段长度多态性（PCR-RFLP）对两组CCL3-2021C/T和CCL4-5725A/C多态性进行基因分型。结果结核组CCL3-2021C/T的CC、CT、TT基因型频率分别为2．5％、15．4％、82．1％，对照组分别为0、18．6％、81．4％，P均＞0．05；结核组C等位基因频率为10．2％、T等位基因频率为89．8％，对照组分别为9．3％、90．7％，P均＞0．05。结核组CCL4-5725A/C的AA、AC、CC基因型频率分别为7．7％、30．8％、61．5％，对照组分别为5．2％、27．8％、67．0％，P均＞0．05；结核组A等位基因频率为23．1％、C等位基因频率为76．9％，对照组分别为19．1％、80．9％，P均＞0．05。结论 CCL3-2021C/T和CCL4-5725A/C多态性与TB易感性不相关。