蜕皮甾酮对2型糖尿病大鼠肝细胞IR S-2蛋白表达的影响

摘要

目的：探讨蜕皮甾酮对2型糖尿病大鼠肝细胞胰岛素受体底物2（ IRS-2）蛋白表达的影响。方法选择42只大鼠，14只作为正常组，常规饲料喂养，余下28只先以高脂喂养加小剂量STZ注射诱导2型糖尿病大鼠模型，造模成功后，随机分为模型组和治疗组，分别给予高脂饲料喂养、高脂饲料喂养＋蜕皮甾酮灌胃治疗。5周后取大鼠肝组织标本，应用免疫组化和RT-PCR技术检测IRS-2蛋白和mRNA含量。结果正常组和治疗组肝细胞IRS-2蛋白及mRNA含量均明显高于模型组（ P＜0．05或＜0．01）。结论蜕皮甾酮可提高2型糖尿病大鼠肝细胞IRS-2蛋白和mRNA含量，这可能是蜕皮甾酮改善2型糖尿病大鼠胰岛素抵抗的机制之一。%Objective To investigate the effect of ecdysterone on the expression of insulin receptor substrate 2 ( IRS-2 ) in liver cell of type 2 diabetic rats .Methods 14 of the 42 rats were randomly selected to the normal control group , and was given ordinary rat food .The other 28 rats were made into type 2 diabetes mellitus rat models by using the high fatty diet and small dose of SIZ , and then diveided into model group and treatment group .The rats in model group or treatment group was given high fatty diet or high fatty diet fecdysterone .IRS-2 protein and mRNA in liver tissues of rats were detected by immunohistochemistry assay and RT-PCR assay after five weeks .Results The expression of IRS-2 protein and mRNA in normal group and treatment group were higher than those of the model group (P<0.01or <0.01).Conclusions Ecdyste-rone led to up-regulation expression of IRS-2 protein and IRS-2 mRNA of liver tissue in diabetic rats .This might play a role in ecdysterone-improved hepatic insulin resistance .