载脂蛋白B基因多态性与激素性股骨头坏死筋脉淤滞证的相关性

摘要

目的：探讨载脂蛋白B （Apo B）基因多态性与激素性股骨头坏死（SONFH）筋脉淤滞证的相关性。方法SONFH 108例患者，中医分型为筋脉瘀滞证64例（筋脉瘀滞组），肝肾亏虚证44例（肝肾亏虚组），另选择因内科疾病使用肾上腺皮质激素治疗半年后未发生SONFH的患者98例为对照组。提取三组血样品DNA，采用直接测序法及PCR-LDR法检测ApoB基因XbaⅠ（ rs693）和EcoRⅠ（ rs1042031）位点的单核苷酸多态性。结果 XbaⅠ位点共检出X-X-和X＋X-两种基因型，筋脉瘀滞组X＋X-基因型及X＋等位基因频率均高于肝肾亏虚组及对照组（P均＜0．05）。 EcoRⅠ位点共检出E＋E＋和E＋E-两种基因型，筋脉瘀滞组E＋E-基因型及E-等位基因频率均高于肝肾亏虚组及对照组（P均＜0．05）。肝肾亏虚组XbaⅠ、EcoRⅠ基因型与等位基因频率分布与对照组相比，差异无统计学意义。结论 ApoB基因XbaⅠ位点的X＋X-基因型、X＋等位基因及EcoRⅠ位点的E＋E-基因型、E-等位基因与SONFH筋脉瘀滞证的发生有关。%Objective To study the relationship between apolipoprotein B ( ApoB) gene polymorphism and the tendon-vessel stagnation of steroid induced osteonecrosis of the femoral head ( SONFH) .Methods A total of 108 patients with SON-FH were included in this study , including 64 patients with tendon-vessel stagnation syndrome of TCM ( tendon-vessel stagna-tion syndrome group ) and 44 patients with the deficiency of liver and kidney syndrome of TCM ( deficiency of liver and kidney syndrome group ) .There were 98 patients who adopt adrenal cortical hormone for treatment in 6 months without SONFH col-lected as control group .DNA of each group were extracted from the blood sample .The direct sequencing polymerase chain re-action and ligase detection reaction (PCR-LDR) technique were used to analyze XbaⅠ(rs693) and EcoRⅠ(rs1042031) single-nucleotide polymorphism of ApoB gene .Results Two gene type, X-X-and X+X-, were detected in XbaⅠsite.The amounts of X+X-genotype and X+allele frequencies of XbaⅠpolymorphism in tendon-vessel stagnation syndrome group were higher than those in deficiency of liver and kidney syndrome group (all P<0.05).Two gene type , which were E+E+and E+E-, were detected in EcoRⅠsite.The amounts of E+E-genotype and E-allele frequencies of EcoRⅠpolymorphism in tendon-vessel stagnation syndrome group were higher than those in deficiency of liver and kidney syndrome group (all P<0.05). However , the difference between the deficiency of liver and kidney syndrome and the control group showed no significance ( P>0.05).Conclusion The XbaⅠX+X-genotype, XbaⅠX+allele frequencies, EcoRⅠE+E-genotype and EcoRⅠE-al-lele frequencies may be related to the tendon-vessel stagnation syndrome of SONFH .