连翘酯苷对顺铂致豚鼠耳毒性的影响及其机制探讨

摘要

目的：探讨连翘酯苷对顺铂致豚鼠耳毒性的影响及其机制。方法30只豚鼠随机分为对照组、顺铂组和连翘酯苷组，每组各10只。顺铂组豚鼠腹腔注射顺铂溶液8 mg/（ kg· d），连续7 d；连翘酯苷组在每次注射顺铂溶液30 min前腹腔注射连翘酯苷25．0 mg/（kg· d），连续7 d；正常对照组以生理盐水代替顺铂溶液腹腔注射，连续7 d。实验动物被处死前，检测其畸变产物耳声发射（ DPOAE）幅值变化；采用免疫组化SP法和Western blot法检测各组豚鼠耳蜗组织bcl-2蛋白的表达。结果1、2、4、6、8 kHz测试频率下，3组DPOAE幅值比较差异均有统计学意义（P均＜0．05），即顺铂组＜连翘酯苷组＜对照组。3组豚鼠耳蜗组织bcl-2蛋白表达比较差异均有统计学意义（P＜0．05或＜0．01），即顺铂组＜连翘酯苷组＜对照组。结论连翘酯苷可减轻顺铂所致耳蜗损伤，上调耳蜗组织bcl-2蛋白表达可能是其作用机制之一。%Objective To study the effect of forsythiaside on cisplatin-induced ototoxicity of guinea pigs .Methods Thirty guinea pigs were randomly divided into the control group (n=10), cisplatin group (n=10) and forsythiaside group (n=10).The guinea pigs in the cisplatin group were induced by intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days, the guinea pigs in the forsythiaside group were given forsythiaside (25 mg/kg per day) at 30 min be-fore cisplatin solution for 7 consecutive days , and the normal saline instead of cisplatin was injected in the control group . The distortion product otoacoustic emission ( DPOAE) was detected before the experimental animals were killed , and the expression of bcl-2 in cochlea of guinea pigs was detected by immunohistochemistry and Western blotting .Results Statis-tical difference was found in the DPOAE amplitudes under 1, 2, 4, 6 and 8 kHz test frequencies among the three groups:cisplatin group