Objective To investigate the expression changes of serum miRNA-34 and miRNA-500 in anti-SSA posi-tive patients with primary Sjogren's Syndrome (pSS)and to analyze their clinical value in diagnosis of anti-SSA positive pSS. Methods Serum samples were obtained from 75 anti-SSA positive patients with pSS (observation group)and 84 an-ti-SSA positive patients without pSS (control group). miRNA microarray was used to determine the alteration of miNRA ex-pression profiles between the above two groups and the results were validated by RT-PCR. The value of miRNAs with abnor-mal expression in diagnosis of anti-SSA positive pSS were analyzed by using statistical methods. Results A total of 10 miRNAs showed altered expression in anti-SSA positive pSS patients,including 6 up-regulated miRNAs and 4 down-regula-ted miRNAs,among which,the most remarkable increase was observed in miR-34 and miR-500 of the observation group. Compared with the control group,the serum miR-34 and miR-500 were significantly increased in the observation group, both P < 0. 001;receiver operating characteristic (ROC)analysis showed that,AUC value of miR-34 in diagnosing anti-SSA positive pSS was 0. 783 (95% CI:0. 710-0. 844,P < 0. 001),and AUC value of miR-500 was 0. 774 (95% CI:0. 701-0. 837,P < 0. 001)in the observation group;AUC value of miR-34 combined with miR-500 in diagnosis of anti-SSA positive pSS was 0. 888 (95% CI:0. 828-0. 932),which was superior to a single detector miR-34 or miR-500 (all P <0. 05). Conclusion The expression of serum miR-34 and miR-500 in anti-SSA positive patients with pSS is increased, and the combined detection of miR-34 and miR-500 is helpful in diagnosis of anti-SSA positive pSS.%目的 观察抗SSA抗体阳性干燥综合征(pSS)患者血清miR-34、miR-500表达变化,并探讨二者在抗SSA抗体阳性pSS诊断中的临床价值.方法 收集pSS患者75例(观察组),非pSS患者84例(对照组),miRNA芯片分析两组血清miRNA表达谱改变;并通过实时荧光定量RT-PCR进行验证.随后应用统计学方法分析异常表达miRNA在抗SSA抗体阳性pSS诊断中的价值.结果 miRNA芯片分析发现6个上调和4个下调的miRNA,其中miR-34和miR-500表达在观察组血清中明显升高;实时荧光定量PCR的结果证实miR-34和miR-500在观察组明显上调,与对照组比较,P<0.001.受试者工作曲线分析结果显示,观察组miR-34对pSS诊断的AUC为0.783(95%CI:0.710~0.844,P<0.001);miR-500的AUC为0.774(95%CI:0.701~0.837,P<0.001).而miR-34联合miR-500在pSS诊断中AUC为0.888(95%CI:0.828~0.932,P<0.001);两者联合诊断pSS的AUC与单一检测比较,P均<0.05.结论 miR-34和miR-500在抗SSA抗体阳性pSS患者血清中表达升高,检测血清miR-34和miR-500有助于抗SSA抗体阳性pSS诊断.
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