T2DM合并冠心病患者与单纯T2DM患者血浆差异表达微小RNA筛选

摘要

目的 筛选2型糖尿病(T2DM)合并冠状动脉粥样硬化性心脏病(简称冠心病)患者与单纯T2DM患者血浆差异表达的微小RNA(miRNA),分析可能用于判断T2DM合并冠心病的潜在生物标记物.方法 选取T2DM患者60例,根据是否合并冠心病分为T2DM合并冠心病组和单纯T2DM组,每组30例.收集两组临床资料,采用微阵列miRNA芯片筛查两组差异表达的miRNA,采用实时荧光定量RT-PCR法检测两组差异表达miRNA的相对表达量(验证微阵列芯片结果),分析差异表达miRNA与T2DM患者临床参数的关系.结果 微阵列miRNA芯片数据筛选出两组存在7个差异表达的候选miRNA,即miR-765、miR-320c、miR-33b-3p、miR-21-5P、miR-22-3p、miR-20b-5p及miR-92a-3p.与单纯T2DM组比较,T2DM合并冠心病组miR-765、miR-320c相对表达量降低(P均<0.01).T2DM患者血浆miR-765、miR-320c相对表达量与HbA1C均呈负相关(β分别为-0.379、-0.192,P均<0.01),与LDL-C均呈负相关(β分别为-0.224、-0.232,P均<0.05).结论 miR-765和miR-320c在T2DM合并冠心病患者与单纯T2DM患者血浆中存在差异表达,二者表达量降低提示T2DM患者有合并冠心病的可能.%Objective To screen out the microRNAs (miRNAs) differentially expressed between type 2 diabetes mellitus (T2DM) patients with and without coronary heart disease.Methods Clinical data were obtained from 30 cases of T2DM patients with coronary heart disease and 30 cases of T2DM patients without coronary heart disease, respectively.miRNA identification was performed by microarray for differentially expressed miRNAs and their expression was confirmed by real-time fluorescence quantification RT-PCR.The relationships between the differentially expressed miRNAs and clinical parameters were analyzed.Results According to the screening of microarray, 7 different miRNAs were selected as candidate miRNAs, including miR-765, miR-320c, miR-33b-3p, miR-21-5P, miR-22-3p, miR-20b-5p and miR-92a-3p.The expression of miR-765 and miR-320c was down-regulated in T2DM patients with coronary heart disease as compared with that of T2DM patients without coronary heart disease (both P<0.01).The expression of miR-765 and miR-320c was negatively correlated with HbA1C (β=-0.379, β=-0.192, both P<0.01) and LDL-C (β=-0.224, β=-0.232, both P<0.05).Conclusion miR-765 and miR-320c express differently between T2DM patients with and without coronary heart disease, which may be the potential biomarkers for T2DM patients with coronary heart disease.