过表达miR-18a-5p对结肠癌细胞增殖和凋亡的影响及其作用机制

摘要

Objective To investigate the effects of miR-18a-5p overexpression on the cell proliferation and apoptosis of colon cancer SW480 cells and its possible mechanism. Methods The colon cancer SW480 cells were randomly divided into the miR-18a-5p-eGFP group, NC-eGFP group, and blank control group. Cells in the miR-18a-5p-eGFP group and NCeGFP group were transfected with miR-18a-5p-eGFP and NC-eGFP plasmid DNA, respectively; the blank control was not transfected. After transfection for 48 h, the cell proliferation inhibition rate was detected by MTT assay, and the early apoptosis rate and late apoptosis rate in each group were detected by flow cytometry. The expression of DUSP5, FZD3, and CCND2 in each group was detected by qRT-PCR. Results At 48 h after transfection, the cell proliferation inhibition rates of miR-18a-5p-eGFP group and NC-eGFP group were (66. 02 ± 0. 51) ％ and (23. 81 ± 0. 64) ％, respectively, and significant difference was found between these two groups (P＜0. 05). The early apoptosis rate and the late apoptosis rate of the miR-18a-5p-eGFP group and NC-eGFP group were both higher than those in the blank control group, and the miR-18a-5peGFP group was higher than the NC-eGFP group (all P＜0. 05). The expression of DUSP5 and FZD3 was higher, while the expression of CCND2 was lower in the miR-18a-5p-eGFP group as compared with that of the NC-eGFP group (all P <0. 05). Conclusion Overexpression of miR-18a-5p can inhibit the proliferation and promote the apoptosis of colon cancer SW480 cells by regulating the expression of target genes DUSP5, FZD3, and CCND2.%目的 探讨过表达miR-18a-5p对结肠癌SW480细胞增殖和凋亡的影响及其可能的作用机制.方法 将结肠癌SW480细胞随机分为miR-18a-5p-eGFP组、NC-eGFP组、空白对照组,miR-18a-5p-eGFP组、NC-eGFP组分别转染含miR-18a-5p-eGFP、NC-eGFP的质粒DNA,空白对照组不予转染.转染48 h,采用MTT法检测各组细胞增殖抑制率,采用流式细胞术检测各组早期凋亡率和晚期凋亡率,采用qRT-PCR法检测各组miR-18a-5p靶基因DUSP5、FZD3、CCND2表达.结果 miR-18a-5p-eGFP组、NC-eGFP组细胞增殖抑制率分别为(66.02±0.51)％、(23.81±0.64)％,两组比较P<0.05.miR-18a-5p-eGFP组、NC-eGFP组早期凋亡率和晚期凋亡率均高于空白对照组,且miR-18a-5p-eGFP组高于NC-eGFP组(P均<0.05).miR-18a-5p-eGFP组miR-18a-5p靶基因DUSP5、FZD3表达明显高于NC-eGFP组,CCND2表达明显低于NC-eGFP组(P均<0.05).结论 过表达miR-18a-5p能够抑制结肠癌SW480细胞增殖并促进其凋亡,其机制可能与调控靶基因DUSP5、FZD3、CCND2表达有关.