京枣试管苗染色体稳定性观察

摘要

以继代培养20代后经生根培养基生根的京枣试管苗为试验材料,用常规染色体压片和显微观察相结合的方法对根尖进行染色体数目观察,结果显示,京枣试管苗的染色体数目2n=24条的占85％,说明经继代培养的京枣试管苗仍保持遗传稳定性.%The test-tube seedlings of Jujube ‘ Jingzao' after rooting culture and 20 generations of subcultures were used as experiment materials.The methods of traditional chromosome tabletting and microscopic observation were adopted to observe the chromosomal number of root tip cell.The results showed that 85％ of the chromosomal number of ‘ Jingzao' was found as 2n =24,which indicated that the test-tube seedlings of ‘ Jingzao' still kept the genetic stability after subculture.