四倍体芦笋花药培养技术初步研究

摘要

The tetraploid asparagus was used to study the effects of bud length and culture conditions on anther culture. The results were as follows. ( 1) When the tetraploid asparagus bud length was 2. 0 ~ 2. 5 mm, the induction effect was the best; (2 ) The callus induction rate was the highest, when the pH value was 6. 0; (3) The end of April was the best inoculation time; (4) The suitable hormone proportion for callus induction was 2, 4 - D 0. 5 mg/L,NAA 0. 5 mg/L and 6 - BA 0. 1 mg/L; (5) The suitable hormone proportion for callus differentiation was 6 - BA 1. 0 mg/L and NAA 0. 5 mg/L.%以四倍体芦笋为试材,研究了花蕾不同长度及培养条件对芦笋花药培养的影响.结果表明:(1)四倍体芦笋花蕾长度为2.0～2.5 mm时,其诱导效果最好；(2)pH值为6.0时愈伤组织诱导率最高；(3)接种时间以4月底最好；(4)适合于四倍体花药愈伤组织诱导的激素配比是2,4-D0.5 mg/L,NAA0.5 mg/L,6-BA 0.1 mg/L；(5)愈伤组织器官分化的培养激素配比是6-BA 1.0 mg/L、NAA 0.5 mg/L.