正交试验法筛选白虎定喘口服液提取工艺研究

摘要

In order to optimize the decoction process of Baihu Dingchuan oral liquid,the extraction process were comprehensively screened by orthogonal test with dry paste volume,contents of chlorogenic acid and baicalin as the evaluation indexes and herbal medicine immersion time,amount of water,decocting time and decocting times as the influence factors.The results showed that decocting times had significant effects on the 3 indicators,and the influences of other factors were not significant.The optimized conditions were adding 15 -fold of water,soaking for 0.5 hour,decocting for 3 times and 0.5 hour each time.The optimized extrac-tion process was stable and feasible with high active ingredient extraction rate,and it could guarantee the qual-ity of preparation.%为了优化白虎定喘口服液的煎煮工艺，以干膏量、绿原酸含量、黄芩苷含量为评价指标，以中药浸泡时间、加水量、煎煮时间、煎煮次数为影响因素，采用正交试验对其提取工艺进行多指标综合筛选。结果显示，煎煮次数对3个指标有显著影响，其他因素影响均不显著；优化的工艺条件为加15倍量水、浸泡0．5 h、每次煎煮0．5 h、煎煮3次。优选出的提取工艺稳定可行，有效成分提取率高，能保证制剂质量。