烯效唑对不同耐旱性甘薯品种苗期根系抗氧化酶同工酶的影响

摘要

The effects of uniconazole on soluble protein content and antioxidant enzyme isoenzymes in seedling roots of JS21 with stronger drought resistance and JZS1 with weaker drought resistance were re-searched by simulating the field drought stress with PEG -6000 in hydroponics.The results showed that under the normal treatment,uniconazole had no significant effects on protein contents of the 2 varieties.Under the drought stress,the soluble protein content of JZS1 increased and the stripe2 ~stripe4 were obviously thick-ened,while that of JS21 decreased and the stripe3 and stripe4 was thinner and lighter.Under the normal treat-ment,uniconazole could increase the isoenzyme expression quantities of SOD2,SOD3 of JS21 and CAT2, CAT3 of JZS1 and had no obvious effects on that of SOD of JZS1,POD of the 2 varieties and CAT of JS21. Compared with the normal treatment,the isoenzyme expression quantities of SOD1,SOD2,POD2 of JZS1 and POD2,POD3 of JS21 increased,that of SOD2 of JS21 decreased under drought stress.Under the drought stress,uniconazole could increase the isoenzyme expression quantities of SOD1,SOD2,POD1,POD3,CAT2 and CAT3.In conclusion,uniconazole could more obviously enhance the isoenzyme expression quantities of antioxidant enzymes in sweetpotato with weaker drought resistance than that with stronger drought resistance under drought stress.%本试验以耐旱性较强的 JS21和耐旱性较差的 JZS1为材料，采用水培和 PEG －6000模拟田间梯度干旱胁迫的方法，研究了烯效唑对不同耐旱性甘薯品种苗期根系可溶性蛋白和抗氧化酶同工酶的影响。结果表明：在正常供水条件下，烯效唑处理对两品种可溶性蛋白含量无明显影响，干旱条件下增加 JZS1的可溶性蛋白含量，stripe2～stripe4明显变粗；降低 JS21的可溶性蛋白含量，stripe3、stripe 4明显变细、变淡。正常供水条件下，烯效唑处理增加 JS21的 SOD2和 SOD3、JZS1的 CAT2和 CAT3同工酶表达量，对 JZS1的 SOD 同工酶表达量、两品种的 POD 同工酶表达量和 JS21的 CAT 同工酶表达量均无明显影响。与正常供水条件相比，干旱处理增加 JZS1的 SOD1、SOD2、POD2和 JS21的 POD2、POD3的同工酶条带的表达量，降低 JS21的 SOD2同工酶表达量。干旱条件下，烯效唑处理增加 JZS1中 SOD1、SOD2、POD1、POD3、CAT2、CAT3同工酶条带的表达量。总之，与耐旱性较强的品种相比，干旱条件下烯效唑处理更能明显增强耐旱性较差的甘薯品种的抗氧化酶同工酶表达量。