黄瓜棒孢叶斑病病菌产毒条件及毒素活性检测

摘要

In order to research the optimum medium and culture time for mycotoxin production by Corynespora cassiicola from cucumber,the strong pathogenic strain HG2 isolated in Tianjin was chosen as tar-get strain.Its bioactivity of crude toxin was detected by seed germination and growth test,leaf wilting degree test,leaf disc test and droplet inoculation test.The results showed that the optimal medium for mycotoxin pro-duction was improved Czapek medium,and the optimal culture time was 21 ~25 days.The seed germination and growth test and leaf wilting degree test were more suitable for activity detection of crude toxin.%为明确黄瓜棒孢叶斑病病原菌多主棒孢的最佳产毒培养基和培养时间，本研究从天津地区采集的黄瓜棒孢叶斑病叶片上分离到强致病菌株 HG2，通过种子萌发生长法、叶片萎蔫法、叶片圆盘法及悬滴接种法4种方法测定其粗毒素的生物活性。结果表明，黄瓜多主棒孢菌最适产毒培养基为改良 Czapek 培养基，培养时间为21～25 d，种子萌发生长法和叶片萎蔫法更适合粗毒素活性测定。