葡萄糖氧化酶和细胞色素C对盐生杜氏藻细胞密度及β－胡萝卜素积累的影响

摘要

Using Dunaliella salina YC01., isolated from Yuncheng Salt Lake, as material, the effects of exogenous glucose-oxidase and cytochrome C on the growth of D.salina andβ-carotene accumulation were investigated.The results showed that the cell density and β-carotene content of D.salina were both maxi-mum when the concentrations of glucose-oxidase and cytochrome C were 9 mg/L and 1 μmol/L, respective-ly.It indicated that the optimal additive concentrations of glucose-oxidase and cytochrome C were 9 mg/L and 1 μmol/L.%为了解外源物质对盐生杜氏藻（ Dunaliella salina）生长和β－胡萝卜素积累的影响，本试验以分离自运城盐湖的一株盐生杜氏藻（ D．salina YC01．）为材料，在培养基中添加外源葡萄糖氧化酶和细胞色素C，研究其对盐生杜氏藻细胞密度和β－胡萝卜素含量的影响。结果表明，葡萄糖氧化酶浓度9 mg／L、细胞色素C浓度1μmol／L时，盐生杜氏藻细胞密度和β－胡萝卜素含量同时最大，因此，为两种外源物质的最优添加浓度。